. 2015 Nov 23;6(4):1230–1241. doi: 10.3390/genes6041230

Table 1.

The primer sequences and PCR-SSCP conditions used for analysis of ovine ADIPOQ.

Region Amplified	Primer Binding Region ^a	Primer Sequence (5'–3')	PCR Annealing Temperature	Amplified Size	SSCP Condition
Region-1	198617873_198617893	F: TTCCTGCTTCTGATCTTGACC	58 °C	388 bp	300 V, 14%, 17 °C
Region-1	198618239_198618260	R: CAGCCTAGAAATTGAATCAGTC	58 °C	388 bp	300 V, 14%, 17 °C
Region-2	198627771_198627789	F: ACAGCGTGGATCTGGGTTC	62 °C	390 bp	200 V, 14%, 32.5 °C
Region-2	198628140_198628159	R: CACAATTCACTTTCGGCTGC	62 °C	390 bp	200 V, 14%, 32.5 °C
Region-3	198628889_198628909	F: GGTCTTCTTGTTCTCTAGGTC	58 °C	391 bp	200 V, 12%, 20 °C
Region-3	198629260_198629279	R: TGGTCCACGTTCTGGTTCTG	58 °C	391 bp	200 V, 12%, 20 °C
Region-4	198629237_198629256	F: TGCTCTTCACCCACGACCAG	58 °C	373 bp	200 V, 14%, 26 °C
Region-4	198629583_198629605	R: GTCCTGCGAACATAGTATATC	58 °C	373 bp	200 V, 14%, 26 °C
Region-5	198629532_198629553	F: GGATTCTGAACATCATTCATTC	58 °C	455 bp	390 V, 14%, 5 °C
Region-5	198629967_198629986	R: CAGATGAGTTGGTGGGAGAC	58 °C	455 bp	390 V, 14%, 5 °C

^a The primer binding position is given relative to the ovine genome sequence (NC_019458.1).