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. 2015 Dec 4;7(12):10189–10208. doi: 10.3390/nu7125527

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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The median and 2.5th and 97.5th percentiles of serum 25(OH)D concentrations by vitamin D supplementation among 11,693 healthy participants of a preventive health program in Canada. Note: The 2.5 percentile line depicts 25(OH)D concentrations for any given vitamin D supplementation dose with 2.5% of all expected 25(OH)D responses to have values below the line, and 97.5% to have values above the line. The 97.5 percentile line depicts 25(OH)D concentrations for any given vitamin D supplementation dose with 97.5% of all expected 25(OH)D responses to have values below the line, and 2.5% to have values above the line. The area between the 2.5 percentile line and the 97.5 percentile line depicts the 95% prediction interval of 25(OH)D concentrations for any given vitamin D supplementation dose. The median, the 2.5th and 97.5th percentiles were estimated through quantile regression, a statistical approach that accommodates the clearly visible skewness in the distribution of 25(OH)D concentrations.