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. 2015 Dec 10;7(12):10388–10397. doi: 10.3390/nu7125537

Figure 7.

Figure 7

(A–D): Apoptotic effect of compound 2 at 10 µg/mL and UNBS1450 at 0.01 µg/mL, 24 h on U937 cells. U937 cells treated with (2) at 10 µg/mL, dimethyl sulfoxide (DMSO) (negative control) or UNBS1450 at 0.01 µg/mL (positive control) were subjected to an Annexin V/PI staining assay. Results were confirmed by three independent analyses, the red squares indicate the cytotoxic effect (A); Hoechst staining of U937 cells treated with DMSO or (2) at 10 µg/mL compared to UNBS1450. Nuclear fragmentation (indicated by red arrows) is observed in cells treated with (2) or UNBS1450 (B); apoptotic effect of (2) at 10 µg/mL or UNBS1450 (positive control) was evaluated by Hoechst staining assay (C), compared to its cytotoxicity evaluated by trypan blue exclusion test (D). Data are the mean ± SD (Standard Deviation) of three independent experiments.