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. 2015 Dec 2;7(12):5236–5253. doi: 10.3390/toxins7124875

Figure 4.

Figure 4

Signal intensity of a 50-attomole injection of each of the three synthetic Stx1 analyte decapeptides (YNDDDTFTVK, YNDDDSFTVK, and YNDDDTFTAK), the ELFTNR, or the ELYTTR peptides in buffer (8% acetonitrile, 0.01% BOG, 25 mM ABC pH 8). Graphs show the MRM signal intensity derived from the y8 ion of the analyte decapeptides or the y4 ions from the other peptides from a (i) 50-attomole injection. The corresponding 15N-labeled analog peptides are normalized to a signal intensity of 500 (ii) to allow the signal from the analyte decapeptide to be viewed on the same plot.