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. 2015 Dec 2;7(12):5236–5253. doi: 10.3390/toxins7124875

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Signal intensity of a 50- or 100-attomole injection of each of the three synthetic Stx1 analyte decapeptides (YNDDDTFTVK, YNDDDSFTVK, and YNDDDTFTAK), the ELFTNR or the ELYTTR peptides in human serum. Graphs show the MRM signal intensity derived from the y₈ ion of the analyte decapeptides or the y₄ ions for the other peptides from a (i) 50- or (ii) 100-attomole injection. The corresponding ¹⁵N-labeled analog peptides are normalized to a signal intensity of 500 (ii) to allow the signal from the analyte decapeptide to be viewed on the same plot.