Figure 7.

A schematic illustration of signal amplified strategy based on target-induced strand release coupling cleavage of nicking endonuclease and its application to OTA detection. In response to the formation of aptamer-OTA complex, the released DNA2 strands from magnetic beads are first hybridized with DNA3, which play a role of PCR template to produce DNA4. Since the PCR template could be recycled by the nicking enzyme, the amount of DN4 was several ten times as much as DNA2 and eventually quantitated with chemiluminescence. (Refer to Ref. [57] for details.)