Figure 5.

BoNT/A-cleaved SNAP25 turn-over at β-Btx-injected NMJ. β-Btx was administered i.m. in mice hind limbs three days after a first injection of BoNT/A at the same site. Immunohistochemistry was then performed at different time points on soleus muscles previously processes for electrophysiology. Similarly to α-Ltx, β-Btx induces an acute degeneration of nerve terminals within 24 h, followed by a complete regeneration. Again, the staining of t-SNAP25 in no more detectable at regenerated motor axon terminals. Bar = 10 µm.