Figure 4.

S. aureus strain, Sa21 grown in BHI under control and MMC induced conditions. Average values including standard deviations (^┬, _┴) of three independent experiments are presented. In MMC induced culture, the point of induction at 4 h is indicated with an arrow. Time is represented in the X-axis in hours (h). (A) Ratio of RF and sea copy levels (Y-axis) as calculated based on Cq values and according to the equation ratio = (E _target) ^{ΔCq target(control-sample)}. Bars represent RF copy levels; dark grey bars designate RF levels for the control culture while light grey bars for the MMC induced culture. The sea₁ gene copy levels are represented by symbols. The levels for the control culture are designated the symbol (▲), while the levels for the MMC induced culture are designated the symbol (×); (B) Ratio of total sea₁ transcript represented in dark grey bars for the control culture and light grey bars for the MMC induced. The cro and long sea₁ transcripts were not detected in either the control or MMC induced cultures.