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Detection of RF by PCR and agarose gel electrophoresis in S. aureus strains Sa17 wild type and Sa17 recA-disruption mutant grown in BHI under induced and controlled conditions: (a) control Sa17 wild type; (b) induced Sa17 wild type; (c) control Sa17 recA-disruption mutant; and (d) induced Sa17 recA-disruption mutant. Positive and negative control are denoted + and −, respectively. DNA ladder mix is included to verify the RF product size.
