Table 3.
Primer sequences used for sequencing the 3.6 kb gene region including the sea gene and for the construction of the recA-disruption mutant.
| Primer | Direction | Sequence | Target DNA | Target Strains |
|---|---|---|---|---|
| YBSEA_1 | Forward | AAGTGGTAAAAAGTTATCTG | 1.5–1 kb upstream sea | MW2, Sa17, Sa21, Sa45, Sa54 |
| YBSEA_2 | Forward | GTAGGCAGTTTTACAACGTC | 1–0.5 kb upstream sea | MW2, Sa17, Sa21, Sa45, Sa54, Sa48, Sa53 |
| YBSEA_3 | Forward | TAAATGGCTCGTAGGTGCCA | 0.5 kb upstream—start of sea | MW2, Sa17, Sa21, Sa45, Sa54, Sa48, Sa53 |
| YBSEA_4 | Forward | ATGAAAAAAACAGCATTTA | Start of SEA—0.5 kb inside sea | MW2, Sa17, Sa21, Sa45, Sa54, MRSA252, Sa48, Sa53 |
| YBSEA_5 | Forward | GTTAAAACGAATAAGAAAAA | 0.5 kb inside SEA—0.2 kb downstream sea | All strains |
| YBSEA_6 | Forward | ATTCCTTATTGCATTGATAG | 0.2–0.7 kb downstream sea | All strains |
| YBSEA_7 | Forward | TATGAAGAACCAAGAAGAAA | 0.7–1.2 kb downstream sea | All strains |
| YBSEA_8 | Forward | CAAGTAAAATAACAGTTGGA | 1.2–1.5 kb downstream sea | All strains |
| YBSEA_9 | Reverse | AATCAATCTCTCATGCCATA | 1.5–1 kb downstream sea | All strains |
| YBSEA_18 | Forward | TCAAACGCTGATAGTGCATA | 2–1.5 kb upstream sea | MW2, Sa17, Sa21, Sa45, Sa54 |
| YBSEA_19 | Forward | CTGATGAGAACTATGATTAC | 2–1.5 kb upstream sea | Newman, Sa51, MRSA252 |
| YBSEA_20 | Forward | GGTTTGATTGCTGAAGAGGT | 1.5–1 kb upstream sea | Newman, Sa51, MRSA252 |
| YBSEA_21 | Forward | AAATCTGAAGAAAACGCTAA | 1–0.5 kb upstream sea | Newman, Sa51, MRSA252 |
| YBSEA_22 | Forward | TGCAGTCATTACTGCATCAA | 0.5 kb upstream—start of sea | Newman, Sa51, MRSA252 |
| YBSEA_101 | Forward | GGAGAAATNGAAGGTATNG | 2–1.5 kb upstream sea | Sa48 |
| YBSEA_105 | Reverse | TATCTAGTGGCTGAAGTAGC | 1.5–1 kb downstream sea | Sa53 |
| YB RecA-108 | Forward | AGTCAGTCGAGCTCTATGGAGAAATCTTTCGGTA | ||
| YB RecA-109 | Reverse | GTCAGTCGCGGCCGCAGTCTCTGGATTACCGAACA | ||
| YB RecA-110 | Forward | CCTTGTATAGTATTGGTAAGATA | ||
| YB RecA-111 | Reverse | GTATCGATAAGCTTGATATC | ||