Distribution of peroxins (A), and possible peroxisomal enzymes (B) in metazoan genomes. Organisms lacking peroxins are highlighted in red. a Identification of peroxins. The green squares indicate identification with high confidence. The identification of Pex10 in the Oikopleura dioica genome with lower confidence (marked by an asterisk) was rejected as a false-positive based on phylogenetic analysis (Figure S1). b Identification of putative peroxisomal enzymes and enyzmes of mitochondrial beta-oxidation. Predicted subcellular localization is indicated in the following colors: grey, no targeting signal detected; red, peroxisomal targeting sequence type 1 or 2 (pts); blue, mitochondrial localization signal (mito); and yellow, secretory pathway signal peptide (sec). Combinations of several different localization signals are shown as follows: violet, pts and mito; orange, pts and sec; green, sec and mito; and black, pts, mito and sec. (Acox1 - Acyl-CoA oxidase1, Acox3 - Acyl-CoA oxidase 3, Lbp - L-bifunctional protein, Dbp - D-bifunctional protein, Acaa1 - Peroxisomal beta-ketothiolase 1, Scp2 - Peroxisomal beta-ketothiolase 2, Amacr - Alpha-methylacyl-CoA racemase, Crat - Carnitine acetyltransferase, Crot - Carnitine octanoyltransferase, Ech1 - Enoyl Coenzyme A hydratase 1, Decr - Peroxisomal 2,4-dienoyl-CoA reductase 2, Pec1 - Peroxisomal 3,2-trans-enoyl-CoA isomerase, Vlcs - Very-long-chain acyl-CoA synthetase, Pte1 - Acyl-CoA thioesterase 2, Pte2 - Acyl-CoA thioesterase 1B, Phyh - Phytanoyl-CoA 2-hydroxylase, Hpcl2 - 2-Hydroxyphytanoyl-CoA lyase, Gnpat - Dihydroxyacetone phosphate acyltransferase, Agps - Alkyldihydroxyacetone phosphate synthase, Far - Fatty acyl-CoA reductase 2, Agxt - Alanine:glyoxylate aminotransferase, Pipox - Peroxisomal sarcosine oxidase/L-pipecolate oxidase, Cat - Catalase, Prdx5 - Peroxiredoxin V, Dao - d-amino acid oxidase, Hao1 - Hydroxyacid oxidase 1, Ephx2 - Epoxide hydrolase, Gstk1 - Glutathione S-transferase class Kappa, Paox - N1-acetylspermine/spermidine oxidase, Xdh - Xanthine dehydrogenase, Uox - Uricase, Allc – Allantoicase)