Table 2.
Data of DMI, milk production, milk composition, and various serum variables related to carbohydrate metabolism as well as to proinflammatory cytokines and acute phase proteins at the diagnosis week, and prior to the diagnosis of subclinical mastitis (SCM) at -8 and -4 weeks prior to expected day of parturition
| -8 weeks before parturation | 4 weeks before parturation | SCM diagnosis weeka | 4 weeks after parturition | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Item | CON | SCM | P-value | CON | SCM | P-value | CON | SCM | P-value | CON P-value | SCM | P-value |
| DMI (kg/d) | 16.27 ± 1.04 | 14.94 ± 1.04 | 0.42 | 15.93 ± 0.10 | 12.98 ± 0.75 | 0.05 | 20.34 ± 0.56 | 16.23 ± 0.44 | <0.01 | 21.16 ± 0.68 | 22.46 ± 1.79 | 0.23 |
| Milk production (kg/d) | 43.01 ± 1.62 | 28.23 ± 3.95 | <0.01 | 42.04 ± 2.21 | 35.36 ± 3.96 | 0.17 | ||||||
| Milk composition (g/kg, unless otherwise stated) | ||||||||||||
| Fat | 5.08 ± 0.45 | 3.32 ± 0.41 | 0.02 | |||||||||
| Protein | 3.00 ± 0.10 | 2.97 ± 0.09 | 0.86 | |||||||||
| Fat : Protein ratio | 1.69 ± 0.12 | 1.11 ± 0.12 | <0.01 | |||||||||
| Lactose | 4.54 ± 0.05 | 4.53 ± 0.06 | 0.92 | |||||||||
| SCC (103 cells/mL) | 28.33 ± 5.63 | 1,867 ± 699 | 0.02 | |||||||||
| Milk urea N (mg/dL) | 15.39 ± 0.76 | 18.70 ± 1.11 | 0.03 | |||||||||
| TS | 12.21 ± 0.31 | 12.19 ± 0.26 | 0.95 | |||||||||
| Serum parameters | ||||||||||||
| Lactate (μmol/L) | 2,455 ± 349 | 3,478 ± 153 | 0.03 | 2,162 ± 184 | 3,467 ± 546 | 0.04 | 2,228 ± 320 | 2,215 ± 203 | 0.98 | 2,100 ± 129 | 2,999 ± 76 | <0.01 |
| NEFA (mmol/L) | 141 ± 32.77 | 179 ± 42.91 | 0.51 | 194 ± 47.17 | 92.97 ± 10.42 | 0.09 | 756 ± 232 | 303 ± 55 | 0.11 | 500.43 ± 151.71 | 243.89 ± 39.28 | 0.16 |
| BHBA (μmol/L) | 352 ± 37.71 | 336 ± 42.62 | 0.78 | 312 ± 18.50 | 354 ± 3.32 | 0.08 | 827 ± 151 | 598 ± 176 | 0.35 | 896.73 ± 188.3 | 777.8 ± 91.43 | 0.58 |
| IL-1 (pg/mL) | 316.79 ± 6.04 | 308 ± 13.76 | 0.55 | 321 ± 1.59 | 306. ± 1.46 | <0.01 | 277. ± 5.42 | 297 ± 4.50 | 0.02 | 270.57 ± 2.87 | 283.02 ± 7.22 | 0.14 |
| IL-6 (pg/mL) | 19.23 ± 5.67 | 13.23 ± 3.15 | 0.42 | 48.24 ± 17.51 | 14.75 ± 6.13 | 0.24 | 23.17 ± 5.18 | 23.43 ± 4.89 | 0.97 | 15.71 ± 3.27 | 21.73 ± 6.02 | 0.38 |
| TNF (ng/mL) | 0.34 ± 0.03 | 0.35 ± 0.02 | 0.96 | 0.27 ± 0.05 | 1.29 ± 0.05 | <0.01 | 0.06 ± 0.03 | 0.23 ± 0.01 | 0.01 | 0.07 ± 0.04 | 0.28 ± 0.04 | 0.01 |
| Haptoglobin (mg/mL) | 0.19 ± 0.03 | 0.05 ± 0.01 | <0.01 | 0.15 ± 0.01 | 0.07 ± 0.01 | <0.01 | 0.12 ± 0.01 | 0.33 ± 0.02 | <0.01 | 0.16 ± 0.01 | 0.10 ± 0.02 | 0.04 |
| SAA (ug/mL) | 8,448 ± 3,373 | 8,799 ± 1,464 | 0.96 | 3,461 ± 342 | 21,052 ± 12,162 | 0.39 | 10,401 ± 1722 | 33,705 ± 9,459 | 0.05 | 11,797 ± 1,853 | 31,419 ± 8,039 | 0.05 |
aCows were diagnosed with subclinical mastitis (n = 6) ranging from week +2 to +3. CON = cows without subclinical mastitis (health control); SCM = cows with subclinical mastitis