Table 1.
Transcriptional changes of the type I IFN signaling pathway in TMEV- compared to mock-infected SJL/J mice
| Gene symbol | 14 dpi | 42 dpi | 98 dpi | 196 dpi |
|---|---|---|---|---|
| Pattern recognition receptors | ||||
| Eif2ak2 (Pkr) | 1.69 | 3.04 | 3.73 | 2.18 |
| Ifih1 (Mda5) | 2.20 | 4.86 | 5.77 | 3.51 |
| Tlr2 | 1.77 | 2.66 | 3.31 | 2.94 |
| Tlr3 | 1.18 | 1.37 | 1.89 | 1.38 |
| Tlr7 | 1.18 | 1.87 | 2.47 | 2.43 |
| Tlr13 | 1.33 | 2.50 | 3.46 | 3.27 |
| Interferon regulatory factors | ||||
| Irf1 | 1.51 | 3.47 | 4.40 | 3.11 |
| Irf3 | 1.07 | 1.06 | 1.09 | 1.18 |
| Irf5 | 1.07 | 1.40 | 1.81 | 1.57 |
| Irf7 | 2.66 | 10.12 | 11.42 | 4.46 |
| Nfkb1 | 1.14 | 1.14 | 1.20 | 1.10 |
| Type I interferons | ||||
| Ifna1 | 1.04 | −1.10 | −1.06 | −1.08 |
| Ifnb1 | 1.02 | 1.07 | −1.08 | −1.11 |
| Type I interferon receptors | ||||
| Ifnar1 | 1.01 | −1.02 | 1.14 | 1.15 |
| Ifnar2 | 1.10 | 1.51 | 1.72 | 1.50 |
| Signal transducers | ||||
| Irf9 | 2.53 | 4.92 | 4.72 | 3.21 |
| Jak1 | 1.01 | 1.02 | −1.04 | 1.06 |
| Socs1 | 1.01 | 1.31 | 1.48 | 1.14 |
| Stat1 | 3.26 | 10.58 | 15.39 | 9.57 |
| Stat2 | 1.39 | 2.49 | 3.30 | 2.03 |
| Tyk2 | 1.15 | 1.12 | 1.32 | 1.19 |
| Classical interferon stimulated genes | ||||
| Isg15 | 3.23 | 11.58 | 15.71 | 6.59 |
| Mx1 | 1.34 | 2.11 | 3.85 | 1.88 |
| Mx2 | 1.24 | 1.61 | 1.76 | 1.31 |
| Oas1a | 2.71 | 7.92 | 11.07 | 6.73 |
| Oas2 | 1.32 | 2.26 | 2.59 | 2.08 |
| Oas3 | 1.01 | 1.59 | 1.75 | 1.23 |
| Oasl1 | 1.61 | 2.81 | 3.65 | 1.99 |
| Oasl2 | 6.70 | 25.78 | 29.16 | 12.93 |
| Rnasel | −1.16 | 1.37 | 1.54 | 1.29 |
| Major histocompatibility (MHC) genes class I/II | ||||
| H2-D1 (MHC-Ia) | 5.58 | 8.03 | 9.78 | 9.42 |
| H2-K1 (MHC-Ia) | 5.42 | 7.45 | 9.70 | 8.56 |
| H2-M3 (MHC-Ib) | 1.62 | 2.80 | 3.48 | 3.25 |
| H2-Q5 (MHC-Ib) | 2.46 | 9.75 | 13.88 | 10.7 |
| H2-Aa (MHC-IIa) | 5.49 | 29.30 | 37.55 | 29.10 |
| H2-Eb1 (MHC-IIa) | 5.11 | 20.11 | 28.18 | 21.00 |
| H2-DMa (MHC-IIb) | 1.31 | 2.93 | 4.23 | 3.22 |
| H2-Oa (MHC-IIb) | 1.25 | 2.78 | 4.39 | 2.79 |
Shown are fold changes at 14, 42, 98, and 196 dpi. Italic type indicates statistically significant up-regulation (P ≤ 0.05)
Legend: Eif2ak2 eukaryotic translation initiation factor 2-alpha kinase 4, H2-Aa H2 antigen A alpha, H2-D1 H2-D region locus 1, H2-DMa H2 antigen locus DMa, H2-Eb1 H2 antigen E beta 1, H2-K1 H2-K region locus 1, H2-M3 H2-M region locus 3, H2-Oa H2-O region alpha locus, H2-Q5 H2-Q region locus 5, Ifih interferon induced with helicase C domain, Ifit interferon-induced protein with tetratricopeptide repeats, Ifna interferon alpha, Ifnb interferon beta, Ifnar interferon (alpha and beta) receptor, Irf interferon regulatory factor, Isg interferon-stimulated protein, Jak Janus kinase, Mx Myxovirus (influenza virus) resistance, Nfkb nuclear factor of kappa light polypeptide gene enhancer in B cells, Oas 2′-5′ oligoadenylate synthetase, Rnasel Ribonuclease L (2′, 5′-oligoisoadenylate synthetase-dependent), Socs suppressor of cytokine signaling, Stat signal transducer and activator of transcription, Tlr Toll-like receptor, Tyk tyrosine kinase