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. 2015 Dec 23;48:69. doi: 10.1186/s40659-015-0062-3

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© Shu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — hAMCs express mesenchymal stem cell specific marker STRO-1 and vimentin. hAMCs were seeded onto 24-well plates and fixed by 4 % paraformaldehyde. After being blocked, cells were incubated with a mouse anti-human vimentin antibody or a mouse anti-human STRO-1 antibody respectively overnight at 4 °C. After being incubated with fluorescence-conjugated secondary antibodies, the cells were observed under a fluorescent microscope. a Expression of mesenchymal marker STRO-1 in hAMCs. b Expression of mesenchymal marker vimentin in hAMCs. Magnification: ×100