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. 2015 Dec 23;15:448. doi: 10.1186/s12906-015-0976-x

Table 5.

Phytochemical constituents of 80 % methanol extract of A. integrifolia

Test Reagents Test result
Alkaloid Wagner’s reagent ++
Terpenoids Chloroform, acetic anhydride, concentrated sulphuric acid ++
Flavonoids Magnesium ribbon, concentrated hydrochloric acid ++
Steroids Chloroform, concentrated H2SO4 +
Tannin 10 % ferric chloride (FeCl3) +
Saponins distilled water ++
Anthraquinone KOH +
Phenol ferric chloride +
Proteins Millions reagent
Carbohydrate Fehling solution, HCl, NaOH
Fats and oils Filter paper +

+++ = very strong positive, ++ = strong positive, + = fair positive, _ = absent