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. 2015 Dec 23;5:73. doi: 10.1186/s13578-015-0052-8

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© Cheng et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The effect of complement C4 on differentiation of T lymphocytes. a Flow Cytometer was used to determine the CD4+CD25+FOXP3+Treg percentage. b The statistical result of Flow Cytometer was shown. c Real-time PCR was used to quantify Foxp3 relative mRNA expression. d TGFβ concentration of cells was determined by ELISA kit