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. 2015 Dec 10;3:e1498. doi: 10.7717/peerj.1498

Figure 3. The effect of volatile anesthetics on molecular aspects of GSIS in MIN6 cells.

Figure 3

MIN6 cells were exposed to the indicated concentration of glucose and isoflurane (iso) or sevoflurane (sev) for 8 h. (A) Caspase 3/7 activity was assayed by the Apo-ONE™ Homogeneous Caspase-3/7 Assay (Promega, Madison, WI, USA) as described in ‘Materials and Methods.’ (B) Cell proliferation was assayed by colorimetric CellTiter 96™ AQueous One Solution Cell Proliferation Assay (Promega Corporation; Madison, WI) as described in ‘Materials and Methods’ ; this contains 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt, and an electron coupling reagent (phenazine ethosulfate). (C) Cells were harvested and the mRNA levels of GLUT2, Cav1.2, and Kir6.2 were assayed by semi-quantitative reverse transcription real-time PCR. Data are presented as the mean ± SD (n = 3).