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. 2015 Dec 24;16:1101. doi: 10.1186/s12864-015-2312-8

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© Zhang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Expression profiles of Cma_004516 in C. maxima. a, RT-PCR analysis of Cma_004516, Cma_004514 and Cma_004515 mRNA in various tissues. R, roots; V, vines; Tv, tip of the vines; L, leaves; F_b, flowers before anthesis; Fa, flowers after anthesis; Fr, young fruits; DAS, days after sowing. P₁, maternal parent Rimu; P₂, paternal parent SQ026; The right side shows the PCR amplification cycles. EF-1A was used as the internal control. b, Analysis of Cma_004516 gene expression in vines by real-time PCR. Each bar represents an average of three independent reactions, including both biological and technical replicates. Error bars indicated SD. c, Analysis of the activities of the Cma_004516 promoters by transient expression in tobacco leaves. a, promoter of the vine type; b, promoter of the bush type