Table 2.

Characteristics of the primers used for RT-qPCR

Gene symbol	Targeted transcript variants	Primer sequences (5′ – 3′)	Amplicon (bp)
TGFB1	NM_000660	(F): TCGCCAGAGTGGTTATCTT	148
		(R): TAGTGAACCCGTTGATGTCC
TGFB2	NM_001135599, NM_003238	(F): ACACTCAGCACAGCAGGGTCCT	80
		(R): TTGGGACACGCAGCAAGGAGAAG
TGFB3	NM_003239	(F): TGAGTGGCTGTTGAGAAGAGA	139
		(R): ATTGTCCACGCCTTTGAATTTGAT
TGFBR1	NM_001130916, NM_004612	(F): GCAGAGCTGTGAAGCCTTGAGA	128
		(R): TGCCTTCCTGTTGACTGAGTTG
TGFBR2	NM_001024847, NM_003242	(F): ATGACATCTCGCTGTAATGC	163
		(R): GGATGCCCTGGTGGTTGA
HPRT1	NM_000194	(F): TGGCGTCGTGATTAGTGATG	160
		(R): GCCTCCCATCTCCTTCATC
GAPDH	NM_001256799, NM_002046	(F): TCTCTGCTCCTCCTGTTC	112
		(R): CGACCAAATCCGTTGACT

F forward primer, R reverse primer, bp base pair