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. 2015 Nov 24;25(1):146–157. doi: 10.1093/hmg/ddv464

Figure 1.

Figure 1.

Acetylated-tubulin level is reduced in mouse Mecp2308/y astrocytes and in human MECP2 p.Arg294* neural progenitors and astrocytes and associated with an increased HDAC6 protein level in Mecp2308/yastrocytes. (A) Acetylated (Ac TUB) and total tubulin (Total TUB) protein expression levels determined by western blot analysis in Mecp2-deficient (Mecp2 308), MECP2 p.Arg294* and wild-type (WT) astrocytes. (B) HDAC6 protein expression level determined by western blot analysis and (C) Hdac6 mRNA level determined by qRT–PCR (right) in Mecp2-deficient (Mecp2 308) and wild-type (WT) astrocytes. Normalization of relative quantification of mRNA or protein levels was based on housekeeping Gapdh gene and protein. Bars represent means ± SEM. *A P-value for Student's test under 0.05, **A P-value under 0.01.