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. 2015 Nov 24;25(1):146–157. doi: 10.1093/hmg/ddv464

Figure 4.

Figure 4.

Lysosome vesicle directionality is altered in mouse Mecp2308/y and human MECP2 p.Arg294* astrocytes and is rescued by EpoD treatment in mouse Mecp2308/y astrocytes. Cells were incubated 1 h with 200 nm LysoTracker® and 10 nm EpoD or vehicle before timelapse imaging. For each vesicle, displacement length (DL) and total track length (TL) were measured by Imaris Software (Bitplane). Vesicle was considered mobile when its total track length was >1 µm and ND when its DL/TL ratio was <20%. We tracked 100–200 vesicles per cell. (A) A total of 55 WT, 64 Mecp2 308/y vehicle-treated cells, 15 WT and 17 Mecp2308/y EpoD-treated cells were analyzed. (B) A total of 57 WT, and 74 MECP2 p.Arg294* cells were analyzed. Bars represent mean proportion of ND mobile vesicles by cell ± SEM.*A P-value for Student's test under 0.05, NS a P-value over 0.05.