Table 2.
Seahorse Bioanalyzer program
| Steps | Whole cells | Permeabilized cells | ||
|---|---|---|---|---|
| 1 | Mix 3 min | Loop (2X) | START
|
|
| Mix 1 min
| ||||
| Wait 3 min | ||||
|
| ||||
| 2 | Loop (3X) | START
|
Loop (3X) | START
|
| Mix 1 min
|
Mix 1 min
|
|||
| Measure 3 min
|
Measure 3 min
|
|||
| Mix 1 min | Mix 30 s | |||
|
| ||||
| 3 | Loop END | Loop END | ||
|
| ||||
| 4 | Inject Port A: Substrate | Inject Port A: 100 μg/ml saponin | ||
|
| ||||
| 5 | Loop (3X) | START
|
Loop (5X) | START
|
| Mix 1 min
|
Mix 1 min
|
|||
| Measure 3 min
|
Measure 3 min
|
|||
| Mix 1 min | Mix 1 min | |||
|
| ||||
| 6 | Loop END | Loop END | ||
|
| ||||
| 7 | Inject Port B: 1 μM oligomycin | Inject Port B: Substrate | ||
|
| ||||
| 8 | Loop (3X) | START
|
Loop (2X) | START
|
| Mix 1 min
|
Mix 1 min
|
|||
| Measure 3 min
|
Measure 3 min
|
|||
| Mix 1 min | Mix 1 min | |||
|
| ||||
| 9 | Loop END | Loop END | ||
|
| ||||
| 10 | Inject Port C: 1 μM FCCP | Inject Port C: 1 μM FCCP | ||
|
| ||||
| 11 | Loop (3X) | START
|
Loop (2X) | START
|
| Mix 1 min
|
Mix 1 min
|
|||
| Measure 3 min
|
Measure 3 min
|
|||
| Mix 1 min | Mix 1 min | |||
|
| ||||
| 12 | Loop END | Loop END | ||
|
| ||||
| 13 | Inject Port D: 5 μM antimycin A | Inject Port D: 5 μM antimycin A | ||
|
| ||||
| 14 | Loop (3X) | START
|
Loop (2X) | START
|
| Mix 1 min
|
Mix 1 min
|
|||
| Measure 3 min
|
Measure 3 min
|
|||
| Mix 1 min | Mix 1 min | |||
|
| ||||
| 15 | Loop END | Loop END | ||