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. Author manuscript; available in PMC: 2015 Dec 24.
Published in final edited form as: Bladder (San Franc). 2015 Jul 17;2(2):e15. doi: 10.14440/bladder.2015.44

Figure 2. Western blot images of HCN 1-4 subtype expression in human (A) and rat bladder (B).

Figure 2

β-Actin was used as a loading control for each lane, where the mucosa containing urothelium and detrusor specimens were added to separate lanes indicated by arrows. A. As predicted by PCR findings, dense band corresponding to HCN4, 110 kDa protein was predominantly detected in human detrusor smooth muscle, with a faint band for HCN4 in urothelium. The bands for HCN1, HCN2 and HCN3 were only detectable in human bladder mucosa with only faint corresponding bands in detrusor. B. In rat bladder, the band corresponding HCN1 subtype was strongest in bladder mucosa with comparable expression of HCN3 and HCN4 in urothelium.