Skip to main content
. Author manuscript; available in PMC: 2015 Dec 24.
Published in final edited form as: Bladder (San Franc). 2015 Jul 17;2(2):e15. doi: 10.14440/bladder.2015.44

Figure 4. Effect of ZD7288 on phasic and neurogenic contractions in the presence of Tetrodotoxin (TTX) (A-C) produced moderate inhibition of EFS evoked contractions at 20 hz frequency.

Figure 4

Three repeated pre-treatment control contractions were evoked with 20hz frequency before incubating strips with ZD7288 10-8 M (A) and 10-4 M (B) repeating the stimulation. TTX 1 μM dramatically reduced the amplitude of EFS contractions 20hz frequency and in presence of TTX 1 μM, ZD7288 at 10-8 M had no discernible effect on the TTX insensitive contractions evoked by EFS (C), notice the different scale compared to (A) and (B). In the presence of TTX 1 μM, ZD7288 produced a dose dependent increase in phasic contraction (D and E), the muscle integral force increased by 217.3 ± 19.2% (n = 3) at the concentration of 10-4 M in myobath relative to pretreatment values. Notice that amplitude of contractions in (C) and (D) is much lower than amplitude of neurogenic contractions measured in absence of TTX shown in (A) and (B).