Skip to main content
. Author manuscript; available in PMC: 2015 Dec 24.
Published in final edited form as: Bladder (San Franc). 2015 Jul 17;2(2):e15. doi: 10.14440/bladder.2015.44

Figure 5. Concentration dependent effect of β3-AR agonist and agents acting on HCN channels on spontaneous activity of rat bladder strips in absence of TTX.

Figure 5

A. Typical traces showing CL316,243 (□) produced concentration dependent decrease in the integrated mechanical activity of rat bladder strips. Relaxant effect of CL316,243 [10-4 M] was decreased in the presence of ZD7288 (Δ) as measured by a doubled mechanical activity from 28.88 ± 2.29% to 51.23 ± 6.83% (*P < 0.05). In contrast, a decrease in mechanical activity caused by CL316,243 was concentration dependently enhanced in the presence of Lamotrigine(∇). Compared to constant concentration of ZD7288 used in combined experiment with CL316,243, in similar experiments involving Lamotrigine, it was added in equimolar concentrations at each concentration addition indicated by arrowhead. B. Lamotrigine induced significant enhancement of detrusor relaxation was most evident at 10-9 M (*P < 0.01). Ordinate Scale is total integrated mechanical activity (above baseline) expressed as a ratio of the pretreatment value (corresponds to 10-10 M on x-axis), which was defined as 100%. Each point in panel B represents mean ± SEM of 3–4 strips.