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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Pulm Pharmacol Ther. 2015 Aug 14;35:87–93. doi: 10.1016/j.pupt.2015.08.003

Figure 5.

Figure 5

Expression and function of a transmembrane adaptor protein Tmem100 in regulating the TRPA1-TRPV1 complex channel activity. A: Tmem100 is expressed in 24% of L4–L6 doral root ganglion (DRG) neurons, predominantly small-diameter cells with an average diameter of 15.7 μm. B: Diagrams showing the relationship of Tmem100 with other DRG markers based upon the data obtained by the double staining technique. C: Mustard oil (MO; 10 μM)-gated whole-cell voltage clamp (holding potential at −60 mV) current densities (IMO) in TRPA1+TRPV1 (1:1)- and TRPA1+TRPV1+Tmem100 (1:1:4)-expressing CHO cells. D: MO (10 μM)-gated whole-cell voltage clamp current densities (IMO) in TRPA1+TRPV1 (1:1)- and TRPA1+TRPV1+Tmem100-3Q (1:1:4)-expressing CHO cells; Tmem100-3Q is a Tmem100 mutant. In both C and D, the number of cells analyzed and those that responded are shown in the parentheses within bars. Data are means ±SEM; ** P < 0.01 (unpaired t-test). (Modified from reference 53)