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. 2015 Dec 24;4:814. doi: 10.1186/s40064-015-1620-4

Fig. 1.

Fig. 1

Hematoxylin-eosin staining of SCC of the EAC showing tumor budding/sprouting (ad) and cytokeratin immunohistochemistry (CK-IHC) for tumor budding/sprouting (eh) at the invasive front. Inset in a, e shows absence of tumor budding; budding grade 0, the others shows presence of tumor budding; b, f shows budding grade 1, c, g shows budding grade 2, and d, h shows budding grade 3