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Expression of immune-related genes in vaccinated channel fish determined by qRT-PCR. Channel catfish were vaccinated twice, at two-week intervals, with PBS, pSCPI, pSCPI + AH, pSCPI + PA, and pSCPI + FIA. Total RNA of the five fish from each group was extracted from the head-kidney 48 h post-challenge and used for qRT-PCR. For each gene, the mRNA level of the PBS-vaccinated fish was set as 1. * indicates significant (p < 0.05) differences.
