Table 1.
Primers used in this study.
| Primer | Sequences (5′→3′) | Target Gene | Product Size (bp) | GenBank |
|---|---|---|---|---|
| 18S F | GGACACGGAAAGGATTGACAGA | 18S rRNA | 121 | AF021880.1 |
| 18S R | GAGGAGTCTCGTTCGTTATCGG | 174 | DQ160229.1 | |
| IL-1β1 F | GCCATGTTGCTAATGTTGTAATCG | IL-1 β | 146 | U77598.1 |
| IL-1β1 R | TGTCTTGCAGGCTGTAACTCTTG | 129 | AJ417565.2 | |
| MHC II F | CGGGAAGGAGATTAAAGGAGGT | MHC II β | 100 | AF053547.1 |
| MHC II R | GTTTGGTGAAGCTGGCGTGT | 122 | NM_001200227.1 | |
| TNF α F | CGCACAACAAACCAGACGAGAC | TNF- α | 122 | GQ179649.1 |
| TNF α R | ACCACTGCATAGATACGCTCGAA | 100 | NM_001200217.1 | |
| MHC I F | GGTATCATCGTTGGTGTAGCCG | MHC I α | 121 | AF021880.1 |
| MHC I R | GGACAGGTTTGAAGCCAGAGTT | 174 | DQ160229.1 | |
| CD4 F | GCAGGGCACGGATAGATGGA | CD4-L2 | 146 | U77598.1 |
| CD4 R | TGGGTTCGCAGAGGCTGATAC | 129 | AJ417565.2 | |
| CD8 F | CCGACAGTGCCTACGACTAAAGC | CD8 α | 100 | AF053547.1 |
| CD8 R | CCAGCAGCCAAAGGAATGAAG | 122 | NM_001200227.1 | |
| IFN F | TGCACGAAGTGAAAGACCAAA | IFN-γ | 122 | GQ179649.1 |
| IFN R | TTAAGGTCCAGCAGCTCAGTGA | 100 | NM_001200217.1 |