Skip to main content
. 2015 Nov 26;16(12):28180–28193. doi: 10.3390/ijms161226093

Figure 3.

Figure 3

Evodiamine activates peroxisome proliferator-activated receptor γ (PPARγ) in PDGF-BB-stimulated VSMCs. (A) VSMCs were pretreated with evodiamine or rosiglitazone for 6 h and then stimulated with PDGF-BB for another 24 h. RT-qPCR was used to measure the mRNA expression levels of PPARγ; (B) Western blot was used to measure the protein levels of PPARγ. The signal ratio of PPARγ to the internal control GAPDH is shown at the bottom; (C) Nuclear and cytoplasmic proteins were extracted separately, and Western blot was used to measure the protein levels of PPARγ in both fractions. The ratio of nuclear/cytoplasmic PPARγ is shown at the bottom. Evo, evodiamine. RSG, rosiglitazone. Data are presented as mean values ± SD of three independent experiments. ** p < 0.01 compared with the control group; ## p < 0.01 compared with the PDGF-BB-stimulated group.