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. 2015 Nov 30;16(12):28311–28319. doi: 10.3390/ijms161226103

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Regulation of pqs quorum sensing by RpoN. (A) Induction of p_pqsA-gfp transcriptional fusion in PAO1 wild-type, ΔrpoN, ΔrpoNCOM, ΔrpoN/pME6032-pqsR and ΔrpoN + PQS (2-heptyl-3-hydroxy-4(1H)-quinolone). Cultures were monitored for their gfp fluorescent protein (GFP) fluorescence by using a Magellen Tecan^® Infinite 200 PRO microplate reader. Means and standard deviations (S.D.) in relative fluorescence units (RFU) from triplicate experiments are shown; (B) High-performance liquid chromatography (HPLC) analysis of HHQ (4-hydroxy-2-heptylquinoline) production by PAO1, ΔrpoN, ΔrpoNCOM, ΔrpoN/pME6032-pqsR, ΔrpoN/pME6032 and ΔpqsR. Means and S.D. from triplicate experiments are shown.