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. 2015 Nov 30;16(12):28311–28319. doi: 10.3390/ijms161226103

Table 1.

Bacterial strains, plasmids and primers used in this study.

Strain(s) or Plasmid Relevant Characteristic(s) Source or Reference
P. aeruginosa strains
PAO1 Prototypic wild-type strain [13]
ΔrpoN Gmr; rpoN derivative of PAO1 constructed by allelic exchange [13]
ΔrpoNCOM Gmr;Tcr; ΔrpoN carrying the pME6031-rpoN vector This work
ΔrpoN/pME6032-pqsR Gmr;Tcr; ΔrpoN carrying the pME6032-pqsR vector This work
ΔrpoN/pME6032-pqsR/ppqsA-gfp Gmr;Tcr; Carbr; ΔrpoN/pME6032-pqsR carrying the ppqsA-gfp vector This work
ΔrpoNCOM/ppqsA-gfp Gmr;Tcr; Carbr; ΔrpoNCOM carrying the ppqsA-gfp vector This work
ΔrpoN/pME6032 Gmr;Tcr; ΔrpoN carrying the pME6032 vector This work
ΔpqsR pqsR derivative of PAO1 constructed by allelic exchange [15]
Staphylococcus aureus
15981 Prototypic wild-type strain [29]
15981/pSB2019 Chlr; 15981 carrying the pSB2019 gfp-expressing vector [29]
Plasmids
pME6031 Tcr; Broad-host-range cloning vector [30]
pME6031-rpoN Tcr; pME6031 carrying the rpoN gene [4]
pME6032 Tcr; broad host range vector [30]
pME6032-pqsR Tcr; pME6032 carrying the pqsR gene [15]
ppqsA-gfp Gmr;Carbr; pUCP22 carrying the pqsA-gfp transcriptional fusion [16]