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. 2015 Dec 2;16(12):28647–28656. doi: 10.3390/ijms161226118

Figure 1.

Figure 1

Induction of recombinant TGIV-MCP and GIV-MCP proteins at different temperatures. After addition of IPTG, the transformed E. coli cultures (A: TGIV-MCP, B: GIV-MCP) were incubated at 18 or 37 °C for 12 h. Bacterial cells were harvested and homogenized. Both soluble and insoluble proteins were subjected to SDS-PAGE (upper panels), followed by Western blotting with anti-His monoclonal antibody (A) or anti-GIV polyclonal antibody (B) (lower panels). C: sample harvested prior to IPTG induction.