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. 2015 Dec 2;16(12):28566–28581. doi: 10.3390/ijms161226120

Figure 1.

Figure 1

PRM workflow: In PRM, a target precursor ion is isolated in the quadrupole analyzer and fragmented in the HCD cell; the fragment ions are then detected in the Orbitrap mass analyzer. PRM generates high resolution, full MS/MS data. During data processing, the MS/MS spectrum is used for the confident identification of the peptide; subsets of fragment ions with highest intensities in the MS/MS spectrum are used for quantification. Peak areas of fragment ions are extracted using <10 ppm mass window and integrated across the elution profile. The Skyline platform supports PRM-based targeted MS quantification [20].