Figure 2.

EZH2 is the direct target of gga-miR-101-3p. DF-1 cells were co-transfected with Luc-EZH2 (3′-UTR) and the indicated RNA oligonucleotides. At 24 h of post-transfection, the cells were assayed for both firefly and renilla luciferase using the dual-luciferase glow assay. Three independent experiments were performed and each sample was tested in triplicates. Data are expressed as means ± SD. Two-tailed Student’s t-test was used to analyze the significant differences (** p < 0.01).