Table 1.
Name | Primer Sequence (5′–3′) | Accession No. |
---|---|---|
Primers for 3′-UTR Cloning | ||
EZH2 3′-UTR-F | GCGCTCGAGCTGCCTTATCTTC | XM 004935061 |
EZH2 3′-UTR-R | ATAGCGGCCGCTAGCTGGTGAGA | XM 004935061 |
Primers for RT-qPCR | ||
GAPDH-F | GAGGGTAGTGAAGGCTGCTG | NM 204305 |
GAPDH-R | CACAACACGGTTGCTGTATC | NM 204305 |
EZH2-F | TGCCTATAATGTACTCATGGTCAC | XM 004935061 |
EZH2-R | TCATCATTGATAAATCCACACTCT | XM 004935061 |
RT-gga-miR-101-3p | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTTCAGTTA | MIMAT0001185 |
gga-miR-101-3p-F | CTGGTAGGGTACAGTACTGTGATA | MIMAT0001185 |
gga-miR-101-3p-R | CTGGTGTCGTGGAGTCGGC | MIMAT0001185 |
gga-5s-rRNA-F | CCATACCACCCTGGAAACGC | |
gga-5s-rRNA-R | TACTAACCGAGCCCGACCCT |