Table 2.
Amino acid composition (mg/g dry weight) of freeze dried A. lecanora and A. lecanora hydrolysates after 24 h of hydrolysis *.
| Amino Acid | A. lecanora | Papain | Alcalase | Bromelain | Flavourzyme | Trypsin | Pepsin |
|---|---|---|---|---|---|---|---|
| Aspartic acid (D) | 78.83 ± 1.56 a | 50.33 ± 0.20 b | 55.96 ± 0.22 b | 53.16 ± 5.60 b | 36.07 ± 3.50 c | 17.27 ± 1.25 d | 49.21 ± 1.60 b |
| Glutamic acid (E) | 106.83 ± 3.90 a | 86.86 ± 1.10 b | 95.63 ± 1.25 b | 87.79 ± 6.74 b | 63.68 ± 4.68 c | 29.90 ± 2.12 d | 89.62 ± 1.21 b |
| Serine (S) | 34.11 ± 0.40 a | 23.03 ± 2.40 c | 27.80 ± 0.71 b | 28.35 ± 0.03 b | 18.85 ± 0.41 d | 7.99 ± 0.60 e | 20.47 ± 0.17 c,d |
| Histidine (H) | 10.93 ± 0.76 a | 4.30 ± 0.02 b,c | 5.88 ± 0.30 b | 4.83 ± 0.83 b,c | 4.55 ± 0.12 b,c | 1.60 ± 0.11 d | 3.58 ± 1.23 c |
| Arginine (R) | 65.98 ± 2.30 a | 45.62 ± 1.83 d | 56.62 ± 0.50 b,c | 58.64 ± 1.12 b | 32.73 ± 1.80 e | 16.55 ± 1.22 f | 54.40 ± 1.06 c |
| Thereonine (T) | 44.13 ± 1.43 a | 29.75 ± 2.60 c | 31.44 ± 0.80 b,c | 35.26 ± 1.18 b | 25.16 ± 1.58 d | 9.77 ± 0.30 e | 29.96 ± 0.72 c |
| Lysine (K) | 45.47 ± 2.10 a | 23.43 ± 4.10 b | 24.85 ± 1.00 b | 20.56 ± 1.26 b,c | 17.25 ± 2.83 c,d | 5.42 ± 0.35 e | 12.73 ± 0.23 d |
| Tyrosine (Y) | 44.14 ± 1.43 a | 29.65 ± 2.60 c | 31.44 ± 0.30 b,c | 35.26 ± 1.60 b | 25.16 ± 1.60 d | 9.77 ± 0.87 e | 29.96 ± 0.72 c |
| Valine (V) | 41.97 ± 1.41 a | 22.72 ± 0.34 d | 33.05 ± 0.60 b | 27.73 ± 0.54 c | 18.65 ± 1.03 e | 10.40 ± 0.17 f | 24.00 ± 0.32 d |
| Methionine (M) | 15.57 ± 0.60 a | 3.27 ± 0.10 c,d | 5.04 ± 0.045 b | 3.29 ± 0.90 c | 3.16 ± 0.30 c,d | 1.31 ± 0.01 e | 2.20 ± 0.18 d,e |
| Cystine (C) | 2.45 ± 0.10 a | 1.17 ± 0.01 c | 1.52 ± 0.25 b | 0.00 ± 0.00 e | 1.40 ± 0.13 b | 0.33 ± 0.06 d | 0.43 ± 0.01 d |
| Isoleucine (I) | 52.36 ± 3.42 a | 10.90 ± 0.20 b | 13.54 ± 0.30 b | 11.54 ± 1.10 b | 10.29 ± 0.12 b | 4.25 ± 0.41 c | 9.73 ± 0.15 b |
| Leucine (L) | 41.97 ± 2.20 b | 32.41 ± 0.31 d | 36.34 ± 2.10 c,d | 38.13 ± 0.81 b,c | 26.16 ± 1.72 e | 12.40 ± 2.01f | 48.22 ± 2.20 a |
| Phenylalanine (F) | 28.68 ± 1.37 a | 10.46 ± 0.30 d | 17.42 ± 0.041 b | 13.70 ± 1.45 c | 10.00 ± 1.28 d | 4.73 ± 0.05 e | 9.03 ± 0.40 d |
| Glycine (G) | 140.63 ± 1.33 a | 107.00 ± 2.02 c | 120.48 ± 4.10 b | 110.75 ± 1.50 c | 53.47 ± 2.40 d | 38.83 ± 3.53 e | 125.58 ± 3.52 b |
| Alanine (A) | 65.09 ± 1.50 a | 50.98 ± 0.87 c | 57.27 ± 1.71 b | 53.25 ± 0.80 c | 27.6 ± 1.52 d | 18.09 ± 0.70 e | 58.99 ± 1.60 b |
| Proline (P) | 59.77 ± 1.47 a | 48.86 ± 0.90 c | 54.64 ± 0.75 b | 50.36 ± 2.10 c | 27.92 ± 0.46 d | 16.91 ± 1.38 e | 57.60 ± 1.41 a,b |
| Total amino acid | 878.91 ± 30.90 a | 579.93 ± 12.40 c | 668.9 2± 10.45 b | 629.60 ± 12.66 b | 402.10 ± 23.24 d | 205.52 ± 15.92 e | 625.71 ± 6.51 b,c |
| Hydrophobic AA | 388.72 | 238.91 | 285.66 | 258.39 | 150.73 | 90.25 | 278.18 |
| Hydrophilic AA | 274.32 | 194.17 | 216.7 | 198.39 | 148.04 | 66.53 | 193.15 |
| Positively charged AA | 120.47 | 69.05 | 81.47 | 79.2 | 49.98 | 21.97 | 67.13 |
* Each value in the table represents the means ± SD of duplicate determinations; a−f Means with the different superscript letters in the same row indicated significant difference within the hydrolysates (p < 0.05).