Figure 2.

Macrophages are major cell types expressing TDAG8 in BAL fluids. (A) Representative cell images of BAL fluids of WT and TDAG8^TP/TP mouse. Arrows represent macrophages. Scale bar is 100 µm; (B) Differential cell counts in BAL fluids. Total, total white blood cells; Neu, neutrophils; Mac, macrophages; Lym, lymphocytes; Eos, eosinophils; and Bas, basophils. Data are mean ± SEM of n = 6 for each group; (C,D) The mRNA expression profile of proton-sensing GPCRs and TLR4 in BAL fluid cells. ^# The expression of TDAG8 was undetectable. Eight to 10 mice were used in each set and data are mean ± SEM of three separate experiments.