Figure 7.

CLZ concentration in the brains of normal and MCAO/reperfusion mice treated with dispersions containing CLZ nanoparticles. MCAO was induced by inserting a silicone-coated 8-0 nylon monofilament into the left middle cerebral artery. Two hours after this procedure, the middle cerebral artery blood flow was restored by withdrawing the nylon monofilament. At 3 h after reperfusion, 0.1 or 0.6 mg/kg CLZ_nano dispersion was injected into the tail vein. Three days after the injection, the brains were removed, and cut 1 mm anterior and 1 mm posterior to the center of the bregma. The sliced brain tissue was divided at the center, and CLZ concentrations in the right (non-infarct site) and left (infarct site) parts were measured. The data are presented as means ± S.E. of seven independent rats. * p < 0.05, vs. normal mice for each category.