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. 2015 Dec 10;16(12):29496–29507. doi: 10.3390/ijms161226179

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of calycosin on the generation of bone resorption pits. (a) BMMs were cultured with indicated dose of calycosin in the presence of M-CSF (20 ng/mL) and RANKL (20 ng/mL) on Corning Osteo Assay Surface 24 well plates. After six days, cells were removed using 5% sodium hypochlorite and staining by Von Kossa method with modified; and (b) the areas were quantified by Image J. The results were expressed as means ± SD from three independent experiments. Scale bar represents 50 μM. * p < 0.05; ** p < 0.01; *** p < 0.001 vs. control.

Effects of calycosin on the generation of bone resorption pits. (a) BMMs were cultured with indicated dose of calycosin in the presence of M-CSF (20 ng/mL) and RANKL (20 ng/mL) on Corning Osteo Assay Surface 24 well plates. After six days, cells were removed using 5% sodium hypochlorite and staining by Von Kossa method with modified; and (b) the areas were quantified by Image J. The results were expressed as means ± SD from three independent experiments. Scale bar represents 50 μM. * p < 0.05; ** p < 0.01; *** p < 0.001 vs. control.