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. 2015 Dec 10;16(12):29496–29507. doi: 10.3390/ijms161226179

Figure 3.

Figure 3

Effects of calycosin on RANKL-induced gene expression. BMMs were cultured with 20 ng/mL of M-CSF and 20 ng/mL of RANKL in the presence or absence of indicated dose of calycosin for one, two, or threedays. The mRNA expression levels of the indicated genes including TRAP, CtsK, and MMP-9 were determined by quantitative real-time PCR. The mRNA levels of the target gene were normalized relative to the mRNA level of β-actin. The results were expressed as means ± SD from three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001 vs. control.