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. 2015 Dec 10;16(12):29467–29481. doi: 10.3390/ijms161226186

Figure 4.

Figure 4

Inhibition of Prmt6 promoter activity by AR along with testosterone treatment in TM4 and COS7 cells. (A,B) TM4 and COS7 cells were transiently cotransfected with 200 ng of pGL4.15 (which has a firefly luciferase reporter gene) or pGL4.15-MMTV (mouse mammary tumor virus long terminal repeat, which was a hormone responsive promoter and located in the upstream of the luciferase reporter gene) or pGL4.15-Prmt6 (containing the promoter region of Prmt6), 200 ng of pcDNA3.1 (empty vector) or pcDNA3.1-Ar (over-expression of Ar, containing the coding sequence of Ar) and treated with or without testosterone (T, 10 nM). The relative luciferase activity was calculated from firefly luciferase data standardized to renilla luciferase data. All the experiments were performed in triplicate and repeated at least three times. Data are expressed as the mean ± SD. * p < 0.05.