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. 2015 Dec 10;16(12):29467–29481. doi: 10.3390/ijms161226186

Figure 5.

Figure 5

The expression of Prmt6 mRNA and PRMT6 protein were down-regulated by AR in TM4 cells. (A,B) TM4 cells were transiently transfected with siNC or siAr, and treated with or without 10 nM testosterone (T), then the expression of Prmt6 mRNA and PRMT6 or AR protein were detected by RT-qPCR and western blotting, respectively. All of the experiments were repeated at least three times. Data are expressed as the mean ± SD. * p < 0.05; (C,D) TM4 cells were transiently transfected with pcDNA3.1 or pcDNA3.1-Ar plasmid and treated with or without 10 nM testosterone. RT-qPCR and western blotting were used to examine the expression of Prmt6 mRNA and PRMT6 or AR protein, respectively. All of the experiments were repeated at least three times. Data are expressed as the mean ± SD. * p < 0.05.