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. 2015 Dec 10;16(12):29467–29481. doi: 10.3390/ijms161226186

Figure 6.

Figure 6

The migratory ability of germ cells is repressed by blocking the function of PRMT6 together with testosterone (T) treatment. Cells were transfected with shNC or shPrmt6 together with or without 10 nM testosterone treatment. (A) Representative photographs of migration assay in GC-1 and GC-2 cells on 0 h (represented the time when the scratch was made) and 12 h are shown; (B) Statistical analysis of the relative migratory ability of GC-1 and GC-2 cells; (C,D) The expression of PRMT6 protein and Prmt6 mRNA in GC-1 and GC-2 cells were examined by western blotting and RT-qPCR, respectively. The expression of Gapdh mRNA and GAPDH protein were used as a loading control. All of the experiments were repeated at least three times. Data are expressed as the mean ± SD. * p < 0.05.