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. 2015 Dec 10;16(12):29467–29481. doi: 10.3390/ijms161226186

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Germ cell apoptosis was promoted by transfecting GC-1 and GC-2 cells with shPrmt6 and treated with testosterone (T). (A) Representative graphs of GC-1 and GC-2 cell apoptosis as analyzed by flow cytometry. LR (lower right section of the graphs) which was Annexin V-FITC positive and PI (propidium iodide) negative represented the percentage of apoptosis cells; (B,C) Statistical analysis of the cell apoptosis rate of GC-1 and GC-2 cells. All of the experiments were repeated at least three times. Data are expressed as the mean ± SD. * p < 0.05.