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. 2015 Dec 15;16(12):29889–29899. doi: 10.3390/ijms161226208

Table 1.

Primer details of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG), sterol-3-β-glucosyl transferase (STRL) and 18S housekeeping genes.

Primer Primer Sequence Reference PE ± SE *
18S Ribosomal RNA F primer: 5′→3′ GTCTCAACCATAAACGATGCCGACCA Accession ID: KJ813746 2.0 ± 0.0
R primer: ACCTGGTAAGTTTCCCCGTGTTGAGT
HMG R primer: 5′→3′ ACTCAACACGGGGAAACTTACCAGGT 171 bp Contig 19639-our transcriptome data 2.1 ± 0.0
F primer: 5′→3′ CACCCGCAAGATGAGATTTT
STRL F primer: 5′→3′ TGGAAAATGAGGATGGGGTA 144 bp Conting 10332-our transcriptome data 2.0 ± 0.0
R primer: 3′→5′TTGCATTGGATCAGGAACAA

* PE ± SE: primer efficiency ± standard error.