Table 1.
Primer details of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG), sterol-3-β-glucosyl transferase (STRL) and 18S housekeeping genes.
| Primer | Primer Sequence | Reference | PE ± SE * |
|---|---|---|---|
| 18S Ribosomal RNA | F primer: 5′→3′ GTCTCAACCATAAACGATGCCGACCA | Accession ID: KJ813746 | 2.0 ± 0.0 |
| R primer: ACCTGGTAAGTTTCCCCGTGTTGAGT | |||
| HMG | R primer: 5′→3′ ACTCAACACGGGGAAACTTACCAGGT | 171 bp Contig 19639-our transcriptome data | 2.1 ± 0.0 |
| F primer: 5′→3′ CACCCGCAAGATGAGATTTT | |||
| STRL | F primer: 5′→3′ TGGAAAATGAGGATGGGGTA | 144 bp Conting 10332-our transcriptome data | 2.0 ± 0.0 |
| R primer: 3′→5′TTGCATTGGATCAGGAACAA |
* PE ± SE: primer efficiency ± standard error.