Table 1.
Summary of transcriptome assembly after Illumina HiSeq 2500 sequencing of Lycaenid butterflies Spindasis takanosis and Protantigius superans.
| Assembly Features | Spindasis takanosis | Protantigius superans |
|---|---|---|
| Raw Reads | ||
| Number of sequences | 249,312,792 | 258,875,070 |
| Number of bases | 31,413,411,792 | 32,618,258,820 |
| Mean length (bp) | 126 | 126 |
| Clean reads | ||
| Number of sequences | 245,110,582 | 254,340,693 |
| Number of bases | 30,515,812,866 | 31,607,701,940 |
| Mean length (bp) | 124.5 | 124.3 |
| N50 length (bp) | 126 | 126 |
| GC% | 41.96 | 39.81 |
| High-quality reads (%) | 98.31 (sequences), 97.14 (bases) | 98.25 (sequences), 96.90 (bases) |
| Number of reads discarded (%) | 1.69 (sequences), 2.86 (bases) | 1.75 (sequence), 3.1 (bases) |
| Contig information | ||
| Total number of contig | 170,449 | 159,074 |
| Number of bases | 134,036,728 | 118,721,203 |
| Mean length of contig (bp) | 786.4 | 746.3 |
| N50 length of contig (bp) | 1372 | 1220 |
| GC% of contig | 38.58 | 38.45 |
| Longest contig (bp) | 16,820 | 15,152 |
| No. of large contigs (≥500 bp) | 66,844 | 62,485 |
| Unigene information- | ||
| Total number of unigenes | 121,140 | 107,950 |
| Number of bases | 100,232,710 | 89,022,313 |
| Mean length of unigene (bp) | 827.4 | 824.7 |
| N50 length of unigene (bp) | 1537 | 1452 |
| GC% of unigene | 38.68 | 38.46 |
| Length ranges (bp) | 124–16,820 | 114–17,062 |