Figure 2.

Initial DNA damage response is not inhibited by 26S proteasome inhibition. (A) SW620 cells were untreated or treated with 10 nM bortezomib, incubated for 24 h, mock-irradiated (0 Gy) or irradiated with 10 Gy, and either immediately placed on ice and prepared for comet assays or incubated for 20 min at room temperature followed by 10 min on ice and prepared for comet assays; (B) Olive tail moments for non-irradiated, irradiated with 10 Gy with no incubation, and irradiated with 10 Gy with incubation were compared for untreated (black) and bortezomib treated (gray) cells. Data for irradiated cells are the average of two independent experiments with error bars denoting standard deviation.