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. 2015 Dec 21;16(12):30405–30421. doi: 10.3390/ijms161226238

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Combination of sub-lethal irradiation and bortezomib or irradiation alone enhances the killing of CRC mediated by CTLs. (A) SW620 cells were treated with 5 Gy, bortezomib or combination of radiation and bortezomib. Cells were harvested and co-incubated with human CEA specific CTLs (E:T ratio 10:1) for 3.5 h at 37 °C in a 96 well plate. The frequency of tumor cells expressing active caspase-3 was determined by flow cytometry and data was analyzed by flowjo software; (B) As a negative control, SW620 cells were treated and incubated under similar condition as described above in the absence of CTLs; (C,D) Bar graph showing the average of two additional replicate experiments. Error bars represent the SEM. * indicates p value <0.05. ** indicate p value <0.005. CEA = Carcinoembryonic antigen and E:T, effector cell to target cell ratio.

Combination of sub-lethal irradiation and bortezomib or irradiation alone enhances the killing of CRC mediated by CTLs. (A) SW620 cells were treated with 5 Gy, bortezomib or combination of radiation and bortezomib. Cells were harvested and co-incubated with human CEA specific CTLs (E:T ratio 10:1) for 3.5 h at 37 °C in a 96 well plate. The frequency of tumor cells expressing active caspase-3 was determined by flow cytometry and data was analyzed by flowjo software; (B) As a negative control, SW620 cells were treated and incubated under similar condition as described above in the absence of CTLs; (C,D) Bar graph showing the average of two additional replicate experiments. Error bars represent the SEM. * indicates p value <0.05. ** indicate p value <0.005. CEA = Carcinoembryonic antigen and E:T, effector cell to target cell ratio.