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. 2015 Dec 17;60(6):835–846. doi: 10.1016/j.molcel.2015.10.023

Figure 2.

Figure 2

Smc5/6 Restriction to G2/M Allows Normal Origin Firing and Replication Fork Speed

(A) BrdU IP-on-chip profile in WT and G2-SMC5 G2-SMC6 cells synchronously released in media containing HU and BrdU. Chromosome 3 is shown with annotated early ARS regions. The p value of the genome-wide overlap between the BrdU clusters in the two strains is indicated.

(B) WT and G2-SMC5 G2-SMC6 were synchronized in G1 and released in S phase in the presence of EdU and HU. Box plot analysis of the interorigin distance (IOD) in the two samples. For each strain, the median (M) value and the number (n) of the IODs analyzed are indicated. The p value, calculated with the Mann-Whitney test, indicates nonsignificant differences between WT and G2-SMC5 G2-SMC6.

(C) Asynchronous WT and G2-SMC5 G2-SMC6 cells were treated with EdU, and cells were collected at 10 and 20 min afterward for molecular combing analysis. Alternatively, WT and G2-SMC5 G2-SMC6 were synchronized in G1 and released in S phase in the presence of EdU and HU for 90 min. Examples of EdU tracks at 10 min and 20 min are reported. The box plot indicates the statistical analysis of the EdU-track length as in (B).

See also Figure S2.